| 一株兼性氧化亚氮还原菌的还原N2O能力 |
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| 引用本文： | 刘春梅,盛荣,刘毅,谌星,魏文学. 一株兼性氧化亚氮还原菌的还原N2O能力[J]. 微生物学报, 2018, 58(8): 1431-1438 |
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| 作者姓名： | 刘春梅 盛荣 刘毅 谌星 魏文学 |
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| 作者单位： | 中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室中国科学院桃源农业生态试验站;中国科学院大学 |
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| 基金项目： | 国家自然科学基金项目（41330856，41501277，41301274）；中国科学院战略性先导科技专项（XDB15020200） |
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| 摘 要： | 【目的】从水稻土中分离筛选出一株兼性氧化亚氮还原菌,并探索其在不同条件下还原N_2O的能力,为减少温室气体N_2O的排放提供重要依据。【方法】通过微生物富集培养分离技术从水稻土中分离得到纯菌;利用nosZ基因和16S rRNA的测序分析鉴定菌株;通过测定菌株在不同条件下N_2O的还原量,分析该菌株还原N_2O的能力及调控因子。【结果】经鉴定,该菌株含有nos Z基因,属于假单胞菌属,在温度30°C、厌氧条件下还原N_2O速率高达0.0219μmol/min以上,改变不同温度和氧气浓度后其能力相对减弱,但仍具备较强的还原N_2O作用。【结论】从水稻土中分离筛选得到的兼性氧化亚氮还原菌为假单胞菌,它在不同环境条件下都具备较强的还原N_2O能力,该菌株可能为减少土壤N_2O排放提供新途径,对保障生态环境安全具有重要的应用价值。
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| 关 键 词： | 氧化亚氮还原菌 假单胞菌 N2O还原能力 nosZ基因 |
| 收稿时间： | 2017-09-30 |
| 修稿时间： | 2017-12-16 |
Capability of N2O reduction of a facultative N2O reducer |
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| Chunmei Liu,Rong Sheng,Yi Liu,Xing Chen and Wenxue Wei. Capability of N2O reduction of a facultative N2O reducer[J]. Acta microbiologica Sinica, 2018, 58(8): 1431-1438 |
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| Authors: | Chunmei Liu Rong Sheng Yi Liu Xing Chen Wenxue Wei |
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| Affiliation: | Key Laboratory of Agro-ecological Processes in Subtropical Regions, Taoyuan Station of Agro-ecology Research, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, Hunan Province, China;University of Chinese Academy of Sciences, Beijing 100049, China,Key Laboratory of Agro-ecological Processes in Subtropical Regions, Taoyuan Station of Agro-ecology Research, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, Hunan Province, China,Key Laboratory of Agro-ecological Processes in Subtropical Regions, Taoyuan Station of Agro-ecology Research, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, Hunan Province, China,Key Laboratory of Agro-ecological Processes in Subtropical Regions, Taoyuan Station of Agro-ecology Research, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, Hunan Province, China;University of Chinese Academy of Sciences, Beijing 100049, China and Key Laboratory of Agro-ecological Processes in Subtropical Regions, Taoyuan Station of Agro-ecology Research, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, Hunan Province, China |
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| Abstract: | |
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| Keywords: | nitrous oxide reducer Pseudomonas sp. N2O-reducing ability nosZ gene |
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