| 西藏拉鲁湿地水体酵母菌多样性及其与理化因子相关性 |
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| 引用本文： | 郭小芳,德吉,龙琦炜,白斌锦,王豪杰,曹亚璞.西藏拉鲁湿地水体酵母菌多样性及其与理化因子相关性[J].微生物学报,2018,58(7):1167-1181. |
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| 作者姓名： | 郭小芳 德吉 龙琦炜 白斌锦 王豪杰 曹亚璞 |
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| 作者单位： | 西藏大学理学院 |
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| 基金项目： | 西藏自治区自然科学基金（2016ZR-15-11）；2016年度西藏大学珠峰学者人才发展支持计划（藏大字[2016]141号） |
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| 摘 要： | 【目的】开展拉鲁湿地水体酵母菌多样性研究,探究理化因子与酵母菌群落结构的相互关系。【方法】采用原位培养法从拉鲁湿地11个水样中分离酵母菌,应用26S r RNA D1/D2区域序列分析,并结合经典分类法对获得的菌株进行分类鉴定,运用SPSS和CANOCO软件分析酵母菌多样性及其与环境因子相关性。【结果】从拉鲁湿地水体中分离得到169株酵母菌,鉴定分属为15个属31个种。优势种为Ustilentyloma graminis和Filobasidium magnum,优势属为Naganishia、Ustilentyloma、Filobasidium和Cystofibasidium。统计分析结果表明,化学需氧量(COD)是影响拉鲁湿地水体酵母菌数量的显著因素,另外,此理化因子是影响Ustilentyloma分布的重要环境条件。【结论】西藏拉鲁湿地酵母菌资源比较丰富,且存在明显的空间异质性。
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| 关 键 词： | 青藏高原 拉鲁湿地 酵母菌 多样性 理化因子 |
| 收稿时间： | 2017/6/22 0:00:00 |
| 修稿时间： | 2017/9/15 0:00:00 |
Spatial dynamics of yeast community and its relationship with environmental factors in Lhalu Wetland, Tibet |
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| Xiaofang Guo,De Ji,Qiwei Long,Binjin Bai,Haojie Wang and Yapu Cao.Spatial dynamics of yeast community and its relationship with environmental factors in Lhalu Wetland, Tibet[J].Acta Microbiologica Sinica,2018,58(7):1167-1181. |
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| Authors: | Xiaofang Guo De Ji Qiwei Long Binjin Bai Haojie Wang and Yapu Cao |
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| Institution: | School of Sciences, Tibet University, Lhasa 850000, Tibet Autonomous Region, China,School of Sciences, Tibet University, Lhasa 850000, Tibet Autonomous Region, China,School of Sciences, Tibet University, Lhasa 850000, Tibet Autonomous Region, China,School of Sciences, Tibet University, Lhasa 850000, Tibet Autonomous Region, China,School of Sciences, Tibet University, Lhasa 850000, Tibet Autonomous Region, China and School of Sciences, Tibet University, Lhasa 850000, Tibet Autonomous Region, China |
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| Abstract: | Objective] We studied the yeast diversity in Lhalu Wetland and analyzed the influence of environmental factors on yeast community. Methods] Yeasts were isolated by in-situ cultivation and analyzed by the D1/D2 domain of large subunit (26S) ribosomal RNA and morphological characterization. Results] In total 169 yeast isolates were identified to 15 genera and 31 species. Ustilentyloma graminis and Filobasidium magnumgenus were the dominating species in the wetland. Moreover, Naganishia, Ustilentyloma, Filobasidium and Cystofibasidium were the dominating genera. COD was significantly positive related to yeast abundance, and it had significant influence on Ustilentyloma. Conclusion] Yeasts were abundant and diverse among various sites in Lhalu Wetland. |
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| Keywords: | Qinghai-Tibet plateau Lhalu Wetland yeast diversity environmental factors |
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