| 基于概率话题模型的轻微型肝性脑病患者肠道菌群结构研究 |
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| 引用本文： | 王侠林,左赞,范红,孔祥阳,贺建峰.基于概率话题模型的轻微型肝性脑病患者肠道菌群结构研究[J].微生物学报,2018,58(7):1274-1286. |
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| 作者姓名： | 王侠林 左赞 范红 孔祥阳 贺建峰 |
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| 作者单位： | 昆明理工大学信息工程与自动化学院;昆明理工大学附属医院云南省第一人民医院消化科 |
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| 基金项目： | 国家自然科学基金（11265007） |
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| 摘 要： | 【目的】利用LDA(Latent Dirichlet Allocation)概率话题模型分析轻微型肝性脑病(MHE)患者服用利福昔明联合益生菌对其肠道菌群结构异质性和临床疗效的影响。【方法】采用R语言包中的LDA概率话题模型的折叠Gibbs抽样蒙特卡洛算法,对MHE患者肠道菌群结构的时间异质性OTUs(operational taxonomic unit)数据集进行分析。【结果】LDA模型将MHE患者的42份粪便样本分成3个主题(topic),并能鉴定出影响MHE患者肠道菌群异质性结构最大的OTUs菌属,分别为埃希菌属(Escherichia)、类杆菌属(Bacteroides)和链球菌属(Streptococcus)。对比治疗前后,这3种菌属在组内的变异模式为同类型菌属的转变次数和频率均高于不同类型的菌属。利福昔明联合益生菌治疗组和单独利福昔明治疗组治疗后,MHE患者的肠道菌群结构均有所改变(P0.05)。此外,根据临床疗效指标,对比两组患者治疗后血清IL-2、IL-4、IL-6、IL-10、TNF-α、TBIL、ALT、CRP、NCT-A、γ-GGT及血氨水平,观察组明显优于对照组,差异显著,有统计学意义(P0.05)。治疗组总有效率88.8%,不良反应总发生率22.2%,对照组总有效率75%,不良反应总发生率38.5%(P0.05)。【结论】LDA模型不仅能有效地量化菌群结构的异质性,还能鉴定出相对应影响异质性最大的OTUs。利福昔明联合益生菌疗法能明显改善MHE患者的血氨水平和血清炎性因子水平,且对MHE患者的肠道菌群结构也有一定的改变,具体表现为致病菌数量减少,有益菌数量增加,具有较好的临床应用价值。
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| 关 键 词： | LDA模型 Gibbs采样 轻微型肝性脑病 利福昔明 利福昔明联合益生菌 |
| 收稿时间： | 2017/8/16 0:00:00 |
| 修稿时间： | 2017/11/5 0:00:00 |
Study of the structure of intestinal microflora in patients with mild hepatic encephalopathy based on probability topic model |
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| Xialin Wang,Zan Zuo,Hong Fan,Xiangyang Kong and Jianfeng He.Study of the structure of intestinal microflora in patients with mild hepatic encephalopathy based on probability topic model[J].Acta Microbiologica Sinica,2018,58(7):1274-1286. |
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| Authors: | Xialin Wang Zan Zuo Hong Fan Xiangyang Kong and Jianfeng He |
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| Institution: | School of Information Engineering and Automation, Kunming University of Science and Engineering, Kunming 650500, Yunnan Province, China,School of Information Engineering and Automation, Kunming University of Science and Engineering, Kunming 650500, Yunnan Province, China;Digestive Department of the First People''s Hospital of Yunnan Province, Medical Faculty of Kunming University of Science and Technology, Kunming 650532, Yunnan Province, China,School of Information Engineering and Automation, Kunming University of Science and Engineering, Kunming 650500, Yunnan Province, China;Digestive Department of the First People''s Hospital of Yunnan Province, Medical Faculty of Kunming University of Science and Technology, Kunming 650532, Yunnan Province, China,School of Information Engineering and Automation, Kunming University of Science and Engineering, Kunming 650500, Yunnan Province, China and School of Information Engineering and Automation, Kunming University of Science and Engineering, Kunming 650500, Yunnan Province, China |
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| Abstract: | |
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| Keywords: | Latent Dirichlet Allocation model Gibbs sampling minimal hepatic encephalopathy rifaximin rifaximin combined with probiotics |
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