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在钙离子敏感性方面与RCH1遗传互作的酿酒酵母基因的筛选
引用本文:王立通,徐慧慧,蒋伶活.在钙离子敏感性方面与RCH1遗传互作的酿酒酵母基因的筛选[J].微生物学杂志,2018(1):34-41.
作者姓名:王立通  徐慧慧  蒋伶活
作者单位:江南大学生物工程学院粮食发酵工艺与技术国家工程实验室;
基金项目:国家自然科学基金项目(81371784,81571966)
摘    要:酿酒酵母ScRCH1是白念珠菌CaRCH1的同功基因,作为人体溶质转运蛋白SLC10A7的同源蛋白,两者都是细胞质膜上钙离子内流的抑制因子。为了研究酿酒酵母RCH1与基因组中其他基因之间的遗传互作,利用合成遗传阵列(Synthetic Genetic Array,SGA)方法构建了RCH1分别与其他非必需基因之间的双基因缺失株文库。钙离子表型筛选表明RCH1与17个基因之间存在遗传互作,其中4个基因BUD9、THR1、RAS2和CPR7在钙离子敏感性方面的功能以前没有报道过。这些结果为深入研究Rch1对钙离子稳态的调控提供了参考。

关 键 词:酿酒酵母  RCH1  合成遗传阵列  钙离子耐受  遗传互作

Genes Screening of Calcium-Ion-Sensitive Aspect and RCH1 Genetic Interaction in Saccharomyces cerevisiae
WANG Li-tong,XU Hui-hui and JIANG Ling-huo.Genes Screening of Calcium-Ion-Sensitive Aspect and RCH1 Genetic Interaction in Saccharomyces cerevisiae[J].Journal of Microbiology,2018(1):34-41.
Authors:WANG Li-tong  XU Hui-hui and JIANG Ling-huo
Abstract:Saccharomyces cerevisiae ScRCH1 is a functional homolog of Candida albicans CaRCH1. As a homologous protein of the human solute carrier SLC10A7, both ScRch1 and CaRch1 are inhibitive factors of calcium uptake in the plasma membrane. In order to investigate the genetic interactions among ScRCH1 and other genes in the genome, in this study double gene deletion mutant library between RCH1 and other nonessential genes were constructed through the synthesis genetic array (SGA) approach. Their calcium sensitive phenotype screen revealed that RCH1 showed genetic interactions with 17 genes, among them BUD9, THR1, RAS2 and CPR7, whose functions in calcium sensitivity were not reported previously. These results provide a basis for further studying on the regulatory mechanism of Rch1 in calcium homeostasis.
Keywords:Saccharomyces cerevisiae  RCH1  synthetic genetic array (SGA)  calcium tolerance  genetic interaction
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