| 羧基荧光素脂质体的制备及其与病原菌的响应行为 |
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| 引用本文： | 孙宁,倪赢,陈仕艳,王华平.羧基荧光素脂质体的制备及其与病原菌的响应行为[J].微生物学通报,2018,45(7):1527-1534. |
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| 作者姓名： | 孙宁 倪赢 陈仕艳 王华平 |
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| 作者单位： | 东华大学纤维材料改性国家重点实验室东华大学高性能纤维及制品教育部重点实验室东华大学材料科学与工程学院 |
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| 基金项目： | 国家自然科学基金(51573024)；中央高校基本科研业务费专项资金；东华大学励志人才计划 |
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| 摘 要： | 【背景】由于目前临床上检测细菌感染的方法既耗时又昂贵,所以开发快速简便的鉴别方法势在必行。【目的】通过在脂质体膜材料中添加不同的稳定剂,研究它们对于脂质体稳定性的影响,并探究荧光素脂质体与病原菌之间的响应情况。【方法】以磷脂酰胆碱和胆固醇为主要原料,1,2-棕榈酰磷脂酰甘油1,2-Dipalmitoyl-sn-glycero-3-phospho-(1?-rac-glycerol),DPPG]、十八胺和10,12-二十三二炔酸(10,12-Tricosadiyonic acid,TCDA)为稳定剂,采用薄膜分散-超声法制备羧基荧光素脂质体。利用病原菌能够分泌毒力因子造成脂质体渗漏的原理,将病原菌菌液和上清液分别与荧光素脂质体孵育,检测从脂质体渗漏的羧基荧光素的荧光强度,反映病原菌的毒性程度。【结果】DPPG和TCDA都能增加脂质体的稳定性,而十八胺的添加则导致脂质体稳定性的降低。与金黄色葡萄球菌ATCC25923和铜绿假单胞菌PAO1菌液和上清液共同孵育的脂质体荧光强度大量增加,与大肠杆菌DH5α和PBS缓冲液共同孵育的脂质体荧光强度几乎不变。【结论】能够分泌外毒素的金黄色葡萄球菌和铜绿假单胞菌都能对脂质体产生响应,而不分泌外毒素的大肠杆菌则不会对脂质体产生响应。
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| 关 键 词： | 羧基荧光素，脂质体，稳定剂，金黄色葡萄球菌，铜绿假单胞菌 |
Preparation of carboxyfluorescein liposomes and their response to pathogenic bacteria |
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| SUN Ning,NI Ying,CHEN Shi-Yan and WANG Hua-Ping.Preparation of carboxyfluorescein liposomes and their response to pathogenic bacteria[J].Microbiology,2018,45(7):1527-1534. |
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| Authors: | SUN Ning NI Ying CHEN Shi-Yan and WANG Hua-Ping |
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| Institution: | State Key Laboratory of Modification of Fiber Materials, Key Laboratory of High Performance Fibers and Products, Ministry of Education, School of Materials Science and Engineering, Donghua University, Shanghai 201620, China,State Key Laboratory of Modification of Fiber Materials, Key Laboratory of High Performance Fibers and Products, Ministry of Education, School of Materials Science and Engineering, Donghua University, Shanghai 201620, China,State Key Laboratory of Modification of Fiber Materials, Key Laboratory of High Performance Fibers and Products, Ministry of Education, School of Materials Science and Engineering, Donghua University, Shanghai 201620, China and State Key Laboratory of Modification of Fiber Materials, Key Laboratory of High Performance Fibers and Products, Ministry of Education, School of Materials Science and Engineering, Donghua University, Shanghai 201620, China |
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| Abstract: | |
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| Keywords: | Carboxyfluorescein Liposome Stabilizing agent Staphyloaoccus aureus Pseudomonas aeruginosa |
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