IL-22基因多态性与中国人群肺结核易感性的相关性研究

本研究旨在探讨白细胞介素22(interleukin 22，IL-22)基因多态性位点与肺结核易感性的关系。采用SNPscan?多重单核苷酸多态性(single nucleotide polymorphism，SNP)分型技术，对453例肺结核患者(结核病组)和373例与患者长期密切接触者〔包括109例潜伏结核感染(latent tuberculosis infection，LTBI)者、264例健康对照者〕的IL-22基因的4个SNP位点(rs1179249、rs2227491、rs17224704、rs2227478)多态性进行分析。结核病组、LTBI组和对照组中4个SNP位点的基因型分布经哈温平衡(Hardy-Weinberg equilibrium，HWE)检验均处于遗传平衡状态。结果显示，rs17224704位点存在AA、TA和TT基因型。＞55岁人群中，此位点TA基因型在对照组中的分布显著高于结核病组(P＝0.047 9，OR＝0.365，95% CI＝0.135～0.991)；AA基因型在LTBI组中的分布显著低于结核病组(P＝0.027 6)；TA基因型在LTBI组中的分布显著高于结核病组(P＝0.007 37，OR＝0.213，95% CI＝0.069～0.660)。对照组和LTBI组的等位基因T频率显著高于结核病组(P＝0.026 9，OR＝0.388，95% CI＝0.167～0.897；P＝0.025 0，OR＝0.322，95% CI＝0.119～0.867)。IL-22基因rs17224704的多态性可能与中国重庆地区汉族人群肺结核易感性显著相关，其等位基因T可能是肺结核的保护基因。

Gene polymorphisms of interleukin 22 and their interaction with susceptibility to tuberculosis in Chinese population

To investigate a potential impact of interleukin 22 (IL-22) gene polymorphisms on susceptibility to pulmonary tuberculosis (PTB) in a Chinese population, a case-control study was conducted in PTB cases (n＝453), latent tuberculosis infection (LTBI) cases (n＝109) and healthy controls (HCs) (n＝264). Genetic polymorphisms of rs1179249, rs2227491, rs17224704, rs2227478 were analyzed by using SNPscan? technique. The genetic frequencies of four single nucleotide polymorphisms (SNPs) met Hardy-Weinberg equilibrium. In the population older than 55, the frequency of genotype TA of rs17224704 SNP was significantly higher in healthy control group than that in PTB group (P＝0.047 9，OR＝0.365，95% CI＝0.135－0.991). The frequency of genotype AA of rs17224704 SNP was significantly lower in LTBI group than that in PTB group (P＝0.027 6). The frequency of genotype TA of rs17224704 SNP was significantly higher in LTBI group than that in PTB group (P＝0.007 37, OR＝0.213，95% CI＝0.069－0.660). The frequency of allele T of rs17224704 SNP was significantly higher in healthy control group and LTBI group than that in PTB group (P＝0.026 9, OR＝0.388, 95% CI＝0.167－0.897; P＝0.025 0, OR＝0.322, 95% CI＝0.119－0.867). The results suggest that allele T of rs17224704 gene in Chinese Han population from Chongqing is probably associated with protection against tuberculosis.