多脂长喙壳菌次生代谢产物的抑菌活性研究

植物病原真菌是生态系统的重要组成部分,能够代谢产生多种生物活性物质。用纸片法检测多脂长喙壳菌(Ceratocystis adiposa)发酵提取物对13种致病细菌的抑菌活性,并对潜在抗菌活性化合物的热、酸碱和光稳定性进行检测,同时应用OSMAC策略寻找多脂长喙壳菌产抗菌化合物的最佳培养条件。结果表明:多脂长喙壳菌提取物对革兰阳性菌——蜡样芽胞杆菌(Bacillus cereus)、缓慢芽胞杆菌(Bacillus lentus)、藤黄微球菌(Micrococcus luteus)具有抗菌活性,MIC分别为6.25、3.125和1.562 5 mg/m L,抗菌化合物具有较好的耐热、耐酸碱和耐辐射性,培养基种类、培养时间和接种量会影响多脂长喙壳菌抗菌化合物的产生,为多脂长喙壳菌抗菌化合物的开发利用提供参考。

Bacteriostatic Activity of Secondary Metabolites of Ceratocystis adiposa

Plant pathogenic fungi are important parts of the ecosystem, they are able to metabolize a variety of bioactive substances. In this study, the antibacterial activity of Ceratocystis adiposa fermented extract on 13 kinds of pathogenic bacteria was screened adopting paper method. The heat, acid base and light stability of potential bacteriostatic active compounds were tested. Meanwhile, OSMAC strategy was used to find the optimum culture conditions for the C. adiposa production of antibacterial compounds. The results showed that C. adiposa extract have antibacterial activity against gram positive bacteria, Bacillus cereus, Bacillus lentus, and Micrococcus luteus, and the MIC values of C. adiposa extract were 6.25, 3.125, 1.562 5 mg/mL respectively. Antibacterial compounds possessed heat resistance, acid base resistance and radiation resistance. The type of media, culture time and inoculum amount could affect the production of antibacterial compounds by C. adiposa. This study has laid the foundation for the development and utilization of antibacterial compounds by C. adiposa.