| 白假丝酵母CFL1基因通过转录调控参与氧化压力应答 |
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| 引用本文： | 彭丽萍,张冰,喻其林,李建荣,梁超,任彤彤,李明春. 白假丝酵母CFL1基因通过转录调控参与氧化压力应答[J]. 微生物学通报, 2018, 45(10): 2209-2216 |
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| 作者姓名： | 彭丽萍 张冰 喻其林 李建荣 梁超 任彤彤 李明春 |
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| 作者单位： | 南开大学微生物学系分子微生物学与技术教育部重点实验室 |
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| 基金项目： | 国家自然科学基金(31670146，81873961，31870139)；天津市自然科学基金(17JCZDJC33300) |
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| 摘 要： | 【背景】CFL1基因是白假丝酵母高铁还原酶基因,介导胞外铁离子的还原,在白假丝酵母胞内铁稳态的维持方面发挥着重要作用。【目的】研究CFL1基因调节氧化压力应答的分子机制。【方法】采用液体培养及巨噬细胞模型,测定CFL1缺失对氧化压力耐受性和杀伤巨噬细胞能力的影响;使用羟基自由基清除剂二甲基亚砜(DMSO)分析其对缓解氧化压力敏感性的影响;采用实时荧光定量PCR分析CFL1缺失对氧化压力应答基因表达的影响;采用过氧化氢酶(CAT)活性测定方法研究CFL1缺失对CAT1基因表达的影响;通过构建WT-CAT1-GFP和cfl1Δ/Δ-CAT1-GFP菌株分析过氧化氢酶基因过表达对cfl1Δ/Δ氧化压力敏感性的影响。【结果】白假丝酵母CFL1基因的缺失会造成杀伤巨噬细胞能力的减弱,氧化压力应答基因表达的下降。过氧化氢酶基因的过表达则能恢复与野生型几乎一致的氧化压力水平。【结论】CFL1基因通过转录调控参与白假丝酵母氧化压力应答过程。
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| 关 键 词： | 白假丝酵母，氧化压力，CFL1基因，转录调控 |
CFL1 regulates oxidative stress response genes in transcriptional levels in Candida albicans |
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| PENG Li-Ping,ZHANG Bing,YU Qi-Lin,LI Jian-Rong,LIANG Chao,REN Tong-Tong and LI Ming-Chun. CFL1 regulates oxidative stress response genes in transcriptional levels in Candida albicans[J]. Microbiology China, 2018, 45(10): 2209-2216 |
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| Authors: | PENG Li-Ping ZHANG Bing YU Qi-Lin LI Jian-Rong LIANG Chao REN Tong-Tong LI Ming-Chun |
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| Affiliation: | Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China,Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China,Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China,Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China,Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China,Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China and Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China |
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