Physical mapping of the Esterase-6 locus of Drosophila melanogaster |
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Authors: | W S Procunier J J Smith R C Richmond |
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Institution: | (1) Department of Entomology, Michigan State University, 48824-1115 East Lansing, MI, USA;(2) Department of Zoology, Michigan State University, 48824-1115 East Lansing, MI, USA;(3) Department of Biology and Office of the Dean, College of Arts and Science, University of South Florida, 33620-5550 Tampa, FL, USA |
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Abstract: | The Esterase-6 gene locus of Drosophila melanogaster although well-characterized, has not been definitly mapped by in situ hybridization. In this paper, a high resolution in situ hybridization protocol using an avidin/biotinylated-horseradish peroxidase/diaminobenzidine system was adopted to refine the physical map position of the Esterase-6 locus. Clarity of signal, detail of banding pattern and absence of background allowed the assignment of a 1.8 kb cDNA encoding Esterase-6 to three bands within subsections 69 A1–A3 on the left arm of polytene chromosome 3. These data refine earlier deletion mapping and low resolution in situ hybridization results, which assigned Esterase-6 to 69 A1–A5. The potential use of this high resolution in situ hybridization technique in the analysis of the physical organization of the Esterase-6 gene duplication and surrounding region is discussed. |
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Keywords: | Drosophila melanogaster Esterase-6 Est-6/Est-P duplication in situ hybridization polytene chromosomes gene mapping |
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