Meiotic metaphase I to telophase II as the most responsive stage during microspore development for callus induction in tomato (<Emphasis Type="Italic">Solanum lycopersicum</Emphasis>) anther cultures |
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Authors: | Email author" target="_blank">José?M?Seguí-SimarroEmail author Fernando?Nuez |
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Institution: | (1) Instituto de Conservación y Mejora de la Agrodiversidad Valenciana (COMAV), Universidad Politécnica de Valencia, Camino de Vera s/n, edificio 9B, 46022 Valencia, Spain |
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Abstract: | The generation of homozygous doubled haploid lines through induction of androgenesis is a promising alternative to the classical
inbreeding and selection programs. However, this technology is poorly developed in tomato, where doubled haploid tomato plants
have only been obtained through anther culture. Despite the fact that anther culture is routinely used in a number of economically
interesting crops, there are still many drawbacks that prevent tomato breeders from adopting this technique, and improvements
in methodology are required. One key issue is the correct identification of the optimal stage for anther excision and culture.
In this paper we characterise in vivo microsporogenesis in tomato, defining the different microspore stages and relating them to the length of the donor flower
bud. In parallel, we cultured anthers of these stages to obtain embryogenic callus, and followed the microscopic development
of the callus contained within the anther. Our data suggest that the stage with the highest response, in terms of callus generation,
is meiosis. In particular, we propose the window from metaphase I to telophase II, including tetrad cellularisation, as the
timeframe where induction can be accomplished in tomato anther cultures. |
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Keywords: | androgenesis anther culture doubled haploids Solanum lycopersicum microspore development |
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