The cell wall of Fusarium oxysporum. |
| |
Authors: | E A Schoffelmeer F M Klis J H Sietsma B J Cornelissen |
| |
Institution: | Institute of Molecular Cell Biology, BioCentrum Amsterdam, Kruislaan 318, Amsterdam, 1098 SM, The Netherlands. schoffelmeer@bio.uva.nl |
| |
Abstract: | Sugar analysis of isolated cell walls from three formae speciales of Fusarium oxysporum showed that they contained not only glucose and (N-acetyl)-glucosamine, but also mannose, galactose, and uronic acids, presumably originating from cell wall glycoproteins. Cell wall glycoproteins accounted for 50-60% of the total mass of the wall. X-ray diffraction studies showed the presence of alpha-1, 3-glucan in the alkali-soluble cell wall fraction and of beta-1, 3-glucan and chitin in the alkali-insoluble fraction. Electron microscopy and lectin binding studies indicated that glycoproteins form an external layer covering an inner layer composed of chitin and glucan. |
| |
Keywords: | |
|
|