| 双价工程菌K99和F41菌毛抗原的提纯、特征分析及免疫效果观察 |
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| 引用本文： | 钟声,李丰生,芮贤良,吕学洗,王庆元,黄培堂. 双价工程菌K99和F41菌毛抗原的提纯、特征分析及免疫效果观察[J]. 中国生物化学与分子生物学报, 1992, 8(2): 190-194 |
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| 作者姓名： | 钟声 李丰生 芮贤良 吕学洗 王庆元 黄培堂 |
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| 作者单位： | 佳木斯医学院生物教研室，军事医学科学院生物工程研究所，军事医学科学院生物工程研究所，佳木斯医学院生物教研室，佳木斯医学院生物教研室，军事医学科学院生物工程研究所 佳木斯 154002，北京 100850，北京 100850，佳木斯 154002，佳木斯 154002，北京 100850 |
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| 摘 要： | 本文利用加热搅拌及Sephadex G-100凝胶过滤方法,从双价重组工程菌RRI(pMG611)中分离了重组K99和F41菌毛抗原。SDS-PAGE测定其分子量,重组K99和F41抗原亚单位分子量分别是17200和29800,与各自野生菌毛亚单位分子量相同。甘露糖抗性血凝试验(MRHA)性质与野生K99和F41抗原相似。双向扩散试验和Western blot分析证实其免疫学性质亦与野生菌毛抗原相似。重组K99和F41抗原的免疫原性较强,能够刺激家兔产生高效价的抗体出现。
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| 关 键 词： | K99菌毛 F41菌毛 免疫原性 |
| 收稿时间： | 1992-04-20 |
Purification and Properties of Recombinant K99 and F41 Fimbriae Antigens From Engineered Strain |
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| Zhong,Sheng Li,Feng-sheng Rui,Xian-liang Lu,Xue-xian Wang,Qing-ynan Huang,Pei-tang. Purification and Properties of Recombinant K99 and F41 Fimbriae Antigens From Engineered Strain[J]. Chinese Journal of Biochemistry and Molecular Biology, 1992, 8(2): 190-194 |
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| Authors: | Zhong Sheng Li Feng-sheng Rui Xian-liang Lu Xue-xian Wang Qing-ynan Huang Pei-tang |
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| Affiliation: | (Molecular Genetics Center,Institute of Biotechnology,Beijing 100850 |
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| Abstract: | The recombinant K99 and F41 fimbriae antigens expressed by E.coli RRI (pMG611) strain had been purified by heat treatment and then by Sephadex G-100 filtratiion.The molecular weight of their subunit were determined by SDS-PAGE.They are the same as those of the corresponding fimbriae antigens from wild strains.The MRHA properties of recombinant antigens are similar to those of wild strains.Immunological characteristics of the recombinant fimbriae were demonstrated by double immunodiffusion test and Western blot assay.They are also similar to those of wild fimbriae.The high titer of antibody of both K99 and F41 reached in rabbit showed that they had strong immunogenicity. |
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| Keywords: | K99 fimbriae F41 fimbriae Purification |
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