A chitinase structurally related to the glycoside hydrolase family 48 is indispensable for the hormonally induced diapause termination in a beetle |
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| Authors: | Fujita Kosuke Shimomura Kumiko Yamamoto Kei-Ichiro Yamashita Tetsuro Suzuki Koichi |
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| Institution: | Department of Agro-Bioscience, Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan. |
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| Abstract: | Two proteins (APAP I and II) of the glycoside hydrolase family 48 (Family GH48) were isolated from the active adults of the leaf beetle Gastrophysa atrocyanea. Full-length and cDNAs were sequenced. APAP I expression and function were examined in detail. The protein has a chitinase but not a glucanase and cellobiohydrolase activity. It is expressed in the feeding stages, including beetles whose diapause was terminated with a juvenile hormone agonist. Suppression of the APAP I expression by means of RNA interference prevented the hormonal termination of diapause. |
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| Keywords: | Glycoside hydrolase GH48 Chitinase Feeding behavior Insect reproductive diapause |
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