| IL-18基因转染对大鼠C6胶质瘤细胞生长特性的影响 |
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| 引用本文： | 蒋常文 闫蕴力 马卫东 郑力芬 翟丽东 李文玲 曹翠丽. IL-18基因转染对大鼠C6胶质瘤细胞生长特性的影响[J]. 细胞生物学杂志, 2005, 27(3): 339-342 |
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| 作者姓名： | 蒋常文 闫蕴力 马卫东 郑力芬 翟丽东 李文玲 曹翠丽 |
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| 作者单位： | 河北医科大学基础医学研究所细胞生物学室 石家庄050017(蒋常文，闫蕴力，马卫东，郑力芬，翟丽东，李文玲)，河北医科大学基础医学研究所细胞生物学室 石家庄050017(曹翠丽) |
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| 摘 要： | 探讨IL-18基因转染对大鼠C6胶质瘤细胞生长特性的影响。用MTT法和流式细胞术检测C6／IL-18细胞和C6细胞的增殖特性和细胞周期分布。免疫细胞化学检测C6／IL-18细胞和C6细胞的增殖细胞核抗原(PCNA)、波形蛋白表达。结果显示，与C6细胞相比C6／IL-18细胞的增殖能力降低，G0/G1期细胞增多而G2／M期细胞减少；PCNA、波形蛋白表达降低。研究表明，IL-18基因具有抑制C6胶质瘤细胞增殖、降低其恶性程度的作用。
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| 关 键 词： | IL-18基因转染 大鼠 C6胶质瘤细胞 生长特性 细胞增殖 基因治疗 |
| 修稿时间： | 2004-09-29 |
The Effects of IL-18 Gene Transfection on the Proliferation of C6 Glioma Cell |
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| Chang-Wen Jiang,Yun-Li Yan,Wei -Dong Ma,Li-Fen Zheng,Li-Dong Zhai,Wen-Ling Li,Cui-Li Cao. The Effects of IL-18 Gene Transfection on the Proliferation of C6 Glioma Cell[J]. Chinese Journal of Cell Biology, 2005, 27(3): 339-342 |
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| Authors: | Chang-Wen Jiang Yun-Li Yan Wei -Dong Ma Li-Fen Zheng Li-Dong Zhai Wen-Ling Li Cui-Li Cao |
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| Affiliation: | Chang-Wen Jiang,Yun-Li Yan*,Wei -Dong Ma,Li-Fen Zheng,Li-Dong Zhai,Wen-Ling Li,Cui-Li Cao |
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| Abstract: | To explore the effects of IL-18 gene transfection on the proliferation of C6 glioma cell. The proliferation and cell cycle distribution of C6/IL-18 and C6 cells were detected with flow cytometry and MTT methods. The expression of PCNA and vimentin were determined with immunocytochemical assay. It was shown that the proliferation of C6/IL-18 cells was reduced compared with its parent C6 cells. The G0/G1 cells were more and G2/M cells became less about C6/IL-18 cells than those of the C6 cells. The expression of the PCNA and vimentin were lower in C6/IL-18 cells than those in C6 cells. It was suggested that IL-18 gene transfection could inhibit the proliferation and decrease malignant degree of C6 cells. |
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| Keywords: | C6 cell line IL-18 gene transfection cell proliferation |
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