The effects of four mycotoxins on the mitogen stimulated proliferation of bovine peripheral blood mononuclear cells in vitro |
| |
Authors: | Charoenpornsook K Fitzpatrick JL Smith JE |
| |
Institution: | (1) Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow, G1 1XW, UK;(2) Department of Veterinary Clinical Studies, University of Glasgow Veterinary School, Glasgow, G61 1QH, UK |
| |
Abstract: | The effects of four mycotoxins, T-2 toxin, deoxynivalenol (DON), ochratoxin A (OTA) and fumonisin B1 (FB1) on the response
of bovine peripheral blood mononuclear cells (PBM) in vitro to the mitogens concanavalin A (Con A), phytohaemagglutinin A
(PHA) and pokeweed mitogen (PWM) were assayed after 4 days' incubation using 3H-thymidine uptake and the MTT bioassay. The
concentrations of mycotoxin required to reduce the proliferative response of PBM by 50% for Con A, PHA and PWM as measured
by 3H-thymidine incorporation was for T-2 toxin 0.30, 0.40 and 0.18 ng ml-1; for DON 0.07, 0.09 and 0.04 μg ml-1; for OTA
0.10, 0.20 and 0.15 μg ml- 1, and for FB1 35, 18 and 11 μg ml-1M by 50% for Con A, PHA and PWM as measured by the MTT bioassay
were for T-2 toxin 2.0, 2.0 and 1.0 mg ml-1; for DON 0.70, 0.50 and 0.50 μg ml-1; OTA 1.5, 1,5 and 1.5 μg ml- 1; and FB1 >50,
>50 and 20 μg ml-1 respectively. Further cytotoxicity assays including the LDH bioassay and Trypan blue exclusion were performed
only on Con A-stimulated PBM cells after 72 h incubation. With the LDH-bioassay the 50% inhibition levels were T-2 toxin 0.3
ng ml-1, DON 0.4 μg ml- 1, OTA 1.4 μg ml-1 and FB1 3.5 μg ml-1; for Trypan blue uptake the 50% inhibition levels were T-2
toxin 5 ng ml-1, DON 2.3 μg ml-1 and OTA 4 μg ml-1 respectively.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | bovine peripheral blood mononuclear cells cytotoxicity deoxynivalenol fumonisin B1 3H-thymidine uptake MTT and LDH bioassays ochratoxin A Trypan blue exclusion T-2 toxin |
本文献已被 PubMed SpringerLink 等数据库收录! |
|