| 硅藻土在青霉素G酰化酶提纯中的应用 |
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| 引用本文： | 任向宇,王建平,张贺迎,吴经才. 硅藻土在青霉素G酰化酶提纯中的应用[J]. 中国生物化学与分子生物学报, 1994, 10(1): 118-121 |
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| 作者姓名： | 任向宇 王建平 张贺迎 吴经才 |
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| 作者单位： | 河北大学生物工程研究所 |
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| 摘 要： | 国产硅藻土经氢氧化铵处理后可用于从发酰液中直接取青霉素Ｇ酰化酶，平均吸附量为９０Ｕ／ｇ。吸附的酶可用２２％硫酸铵－０．３ｍｏｌ／ＬＰＢＳ（ｐＨ８．０）溶液洗脱。平均比１８Ｕ／１８Ｕｍｇ蛋白（ＮＩＰＡＢ法）。硅藻土可反复使用。苯乙酰胺－Ｓｅｐｈａｒｏｓｅ４Ｂ树脂可对酶作进一步的纯化。
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| 关 键 词： | 青霉素G酰化酶；纯化；硅藻土 |
| 收稿时间： | 1994-02-20 |
The Application of Celite Treated by NH_4OH for the Purification of Penicillin m Acylase from Bacillus Megaterium |
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| Ren Xiang-yu,Wang Jian-ping,Zhang He-ying,Wu Jing-cai. The Application of Celite Treated by NH_4OH for the Purification of Penicillin m Acylase from Bacillus Megaterium[J]. Chinese Journal of Biochemistry and Molecular Biology, 1994, 10(1): 118-121 |
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| Authors: | Ren Xiang-yu Wang Jian-ping Zhang He-ying Wu Jing-cai |
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| Affiliation: | (Institute of Biotechnology, Hebei University, Baoding,071002 |
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| Abstract: | The penicillin G-acylase(PGA) from Bacillus megaterium fermenting solution has been obtained directly by using dornestic celite which has been treated by NH4OH. The average adsorbance is about 100U/g,PGA adsorbed by the treated celite can be eluted by 22% saturated ammonium sulfate in 0.3mol/L phosphate buffer(pH=8.0).The average specific activity is 18U/mg protein. The treated celite may be used repeately. Passing through the phenylacetamide-sepharose 4B, PGA has been purified further. |
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| Keywords: | Penicillin G-acylase Purification Celite |
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