AFM studies of water-soluble wheat arabinoxylans--effects of esterase treatment |
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Authors: | Adams Elizabeth L Kroon Paul A Williamson Gary Morris Victor J |
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Institution: | Institute of Food Research, Norwich Research Park, Colney Lane, Norwich NR4 7UA, UK. |
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Abstract: | The degradation products of water-soluble wheat arabinoxylans treated with Aspergillus niger ferulic acid esterase (FAEA-able to cleave 5,5'- and 8-O-4'-ferulic acid dimers) have been characterised by atomic force microscopy (AFM) and size exclusion chromatography. The AFM images of arabinoxylans confirmed that a small proportion ( approximately 15%) of the population of arabinoxylan molecules contain xylan-based branches attached to the xylan-based backbone. Treatment with FAEA reduced the contour length of the molecules suggesting that certain dimeric ferulic acid linkages may play a previously unconfirmed role in the elongation of arabinoxylans. Overnight treatment with FAEA led to a reduction in the density of branches suggesting that they may also be linked to the backbone through phenolic linkages. |
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Keywords: | Arabinoxylans Pentosans Atomic force microscopy Ferulic acid esterase |
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