Refined Analysis of Brain Energy Metabolism Using In Vivo Dynamic Enrichment of 13C Multiplets |
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| Authors: | Masoumeh Dehghani M. Bernard Lanz Jo?o M. N. Duarte Nicolas Kunz Rolf Gruetter |
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| Affiliation: | 1Laboratory for Functional and Metabolic Imaging (LIFMET), Ecole Polytechnique Fédérale de Lausanne (EPFL), Switzerland;2Department of Radiology, University of Lausanne (EPFL), Switzerland;3CIBM-AIT, Ecole Polytechnique Fédérale de Lausanne, Switzerland;4Department of Radiology, University of Geneva, Switzerland |
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| Abstract: | Carbon-13 nuclear magnetic resonance spectroscopy in combination with the infusion of 13C-labeled precursors is a unique approach to study in vivo brain energy metabolism. Incorporating the maximum information available from in vivo localized 13C spectra is of importance to get broader knowledge on cerebral metabolic pathways. Metabolic rates can be quantitatively determined from the rate of 13C incorporation into amino acid neurotransmitters such as glutamate and glutamine using suitable mathematical models. The time course of multiplets arising from 13C-13C coupling between adjacent carbon atoms was expected to provide additional information for metabolic modeling leading to potential improvements in the estimation of metabolic parameters.The aim of the present study was to extend two-compartment neuronal/glial modeling to include dynamics of 13C isotopomers available from fine structure multiplets in 13C spectra of glutamate and glutamine measured in vivo in rats brain at 14.1 T, termed bonded cumomer approach. Incorporating the labeling time courses of 13C multiplets of glutamate and glutamine resulted in elevated precision of the estimated fluxes in rat brain as well as reduced correlations between them. |
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| Keywords: | 13C isotopomers, brain energy metabolism, in vivo13C NMR spectroscopy, metabolic modeling, neuronal/glial compartment |
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