Effects of pulsed electric fields on DNA of human lymphocytes |
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| Authors: | J Delimaris S Tsilimigaki N Messini-Nicolaki E Ziros S M Piperakis |
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| Institution: | (1) DNA Repair Laboratory, Institute of Biology, National Center for Scientific Research “Demokritos”, Athens, Greece;(2) Department of Cell Biology, School of Biology, University of Athens, Greece;(3) Laboratory of Microbiology, 1st I.K.A. Health Department, Athens, Greece;(4) Biology Unit, Department of Pre-School Education, University of Thessaly, Argonafton and Filellinon Streets, Volos, 38221, Greece |
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| Abstract: | The effects of pulsed electric fields of low frequency (50 Hz) on DNA of human lymphocytes were investigated. The influence
of additional external factors, such as hydrogen peroxide (H2O2) and γ-irradiation, as well as the repair efficiency in these lymphocytes, was also evaluated. The comet assay, a very sensitive
and rapid method for detecting DNA damage at the single cells level was the method used. A significant amount of damage was
observed after exposure to the electric fields, compared to the controls. After 2 h incubation at 37°C, a proportion of damage was repaired. H2O2 and γ-irradiation increased the damage to lymphocytes exposed to pulsed electric fields according to the dose used, while
the amount of the repair was proportional to the damage. |
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| Keywords: | comet assay DNA damage humans pulsed electric fields |
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