Sperm dimorphism in terms of nuclear shape and microtubule accumulation in Cyrtanthus mackenii |
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Authors: | Tomonari Hirano Yoichiro Hoshino |
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Institution: | (1) Division of Innovative Research, Creative Research Initiative ‘Sousei’ (CRIS), Hokkaido University, Kita 21, Nishi 10, Kita-ku, Sapporo 001-0021, Japan;(2) Field Science Center for Northern Biosphere, Hokkaido University, Kita 11, Nishi 10, Kita-ku, Sapporo 060-0811, Japan; |
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Abstract: | Pollen tubes of Cyrtanthus mackenii, a species with bicellular pollen, were cultured in vitro to investigate nuclear phase changes during generative cell division
and male germ unit (MGU) formation, using flow cytometric analysis. Results revealed that sperm cells were formed after 12 h
of culture. During sperm maturation, the nuclei of sperm cells were not associated with the vegetative nucleus (unassociated
sperm cells; Sua) and became longer than those of sperm cells associated with the vegetative nucleus (Svn). These findings
indicate that the pair of sperm cells in the C. mackenii MGU is dimorphic in terms of nuclear shape. Dimorphism coincides with anti-α-tubulin antibody immunofluorescence, which was
higher in the Sua than in Svn. Following treatment with oryzalin, triggering microtubule depolymerization, differences between
nuclear shapes in the two sperm nuclei disappeared, suggesting that microtubule accumulation between sperm cells in the MGU
correlates with differences in the nuclear shape. |
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