Differentiation between clathrin-dependent and clathrin-independent endocytosis by means of membrane fluidity measurements |
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Authors: | Gilliane Coupin Jean-Georges Kuhry |
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Institution: | (1) Laboratoire d'Immunopharmacologie, URA 491, Centre National de la Recherche Scientifique, Université Louis Pasteur, Strasbourg, France;(2) Laboratoire de Biophysique, URA 491, Centre National de la Recherche Scientifique, Université Louis Pasteur, Strasbourg, France |
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Abstract: | The fluorescence probe 1-(4-trimethylammonium]-6-phenyl-1,3,5-hexatriene (TMA-DPH) displays properties relevant for both
monitoring endocytosis kinetics and assessing membrane fluidity by fluorescence-anisotropy measurements (1). Thus, it is, possible with this probe to follow the evolution of membrane fluidity during endocytosis, from the very beginning
of the process, i.e., the formation of endocytic vesicles. In most cases, endocytosis is known to start with clathrin-coated
vesicles. Still, there are more and more arguments in favor of a complementary endocytic pathway without clathrin. In this
article we present membrane-fluidity data for very early endocytosis, which allow an upper limit to be determined for the
contribution of a putative nonclathrin pathway. We show that this limit is markedly higher for bone marrow-derived macrophages
than for mouse fibroblasts of the L929 cell line. |
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