In vitro transformation of ampicillin to cephalexin by free and immobilized cells ofStreptomyces sp. |
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Authors: | D. Roy A. Guha M. K. Roy A. C. Ghosh |
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Affiliation: | (1) Biochemistry Division, Regional Research Laboratory, 785 006 Jorhat, Assam, India |
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Abstract: | In vitro transformation of ampicillin to cephalexin was studied using calcium alginate-immobilized and freeStreptomyces sp. strain DRS-1 packed in glass columns. Tris-HCl buffer containing ampicillin was continuously circulated through the columns for four cycles, each cycle (with fresh ampicillin) being continued for 5 h. The pattern of product formation was identical in both cases,i.e. in each cycle, after reaching a certain concentration, its formation did not increase. Product formation was always higher with immobilized cells. Conversion of ampicillin to cephalexin by the strain was affected by cell and substrate concentration. |
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