Clonogenic survival of human keratinocytes and rodent fibroblasts after irradiation with 25 kV x-rays |
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Authors: | Panteleeva Anna Słonina Dorota Brankovic Katarina Spekl Kathrin Pawelke Jörg Hoinkis Cordelia Dörr Wolfgang |
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Affiliation: | (1) Institute of Nuclear and Hadron Physics, Forschungszentrum Rossendorf, P.O. Box 510119, 01314 Dresden, Germany;(2) Laboratory of Radiation Biology, Centre of Oncology, Kraków, Poland;(3) Medical Faculty Carl Gustav Carus, Technical University Dresden, Germany |
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Abstract: | Low energy x-rays (Eph 50 keV) are widely used in diagnostic radiology and radiotherapy. However, data on their relative biological effectiveness (RBE) are scarce. Of particular importance for risk estimation are the RBE values of x-rays in the range which is commonly used in mammography (10–30 keV). We have determined clonogenic survival after low-energy x-ray irradiation for three cell lines: primary human epidermal keratinocytes (HEKn), mouse fibroblasts (NIH/3T3) and Chinese hamster fibroblasts (V79). Experiments were performed with a 25 kV x-ray tube and compared to 200 kV x-rays as a reference. Compared to the effect of 200 kV x-rays, irradiation with 25 kV x-rays resulted in a decreased survival rate in the murine fibroblasts, but not in the human epithelial cell line. The RBE value was calculated for 10% surviving fraction. For HEKn cells, RBE was 1.33±0.27, for NIH/3T3 cells 1.25±0.07 and for V79 cells 1.10±0.09, respectively. No consistently increased RBE was observed in the various cell lines. Nevertheless, a potential of increased cytogenetic changes has to be considered for risk estimation of low-energy x-rays. |
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