| 2株产纤维素酶放线菌的筛选及分类鉴定 |
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| 引用本文： | 黄惠琴,黄美容,叶建军,朱军,鲍时翔. 2株产纤维素酶放线菌的筛选及分类鉴定[J]. 微生物学杂志, 2010, 30(1): 47-50 |
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| 作者姓名： | 黄惠琴 黄美容 叶建军 朱军 鲍时翔 |
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| 作者单位： | 中国热带农业科学院,热带生物技术研究所/农业部热带作物生物技术重点开放实验室,海南,海口,571101 |
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| 基金项目： | 国家863重点项目，国家863计划 |
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| 摘 要： | 从海南热带生境中采集样品14份,以CMC-Na为唯一碳源的培养基,筛选获得纤维素酶活性较高的放线菌DA08506和DA08602,CMC酶活力分别为276.642U/mL、773.982U/mL,FPA酶活力分别为82.638U/mL、325.674U/mL。通过多相分类,初步鉴定菌株DA08506可能是Streoptomyces的一个新种;菌株DA08602鉴定为Streptomyces chartreusis。
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| 关 键 词： | 纤维素酶 放线菌 筛选 分类鉴定 16S rRNA基因 |
Screening and Identification of Two Cellulase-producing Actinomycetes |
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| HUANG Hui-qin,HUANG Mei-rong,YE Jian-jun,ZHU Jun,BAO Shi-xiang. Screening and Identification of Two Cellulase-producing Actinomycetes[J]. Journal of Microbiology, 2010, 30(1): 47-50 |
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| Authors: | HUANG Hui-qin HUANG Mei-rong YE Jian-jun ZHU Jun BAO Shi-xiang |
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| Affiliation: | HUANG Hui-qin,HUANG Mei-rong,YE Jian-jun,ZHU Jun,BAO Shi-xiang(Inst.of Tropical Biosci., Biotech./Key Open Lab.of Tropical Crop Biotech.of Agric.Minist.,Chinese Acad.of Tropical Agric.Sci.,Haikou 571101) |
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| Abstract: | 14 samples were collected from tropical environments in Hainan province,and producing-cellulase strains DA08506 and DA08602 were screened with CMC-Na as a sole carbon source medium.Their CMCase activities were 276.642 U and 773.982 U,and FPA were 82.638 U and 325.674 U,respectively.Strain DA08506 was likely a novel species of genus Streptomyces using polyphase classification.And strain A08602 was identified as Streptomyces chartreusis. |
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| Keywords: | cellulase Actinomyces screening identification 16S rRNA gene |
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