| 内皮素-1 mRNA反义寡核苷酸预防大鼠急性缺血性心律失常 |
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| 作者姓名： | Lin L Yuan WJ Chu XL Xu H Li L Ren AJ |
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| 作者单位： | 第二军医大学生理学教研室!上海,200433,第二军医大学生理学教研室!上海,200433,解放军福州医学高等专科学校生理学教研室!福州,350003,第二军医大学生理学教研室!上海,200433,第二军医大学生理学教研室!上海,200433,第二军医大学生理学教研室!上海,200433 |
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| 基金项目： | 国家自然科学基金!39770855,“九五”全军医学科研基金!96M072 |
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| 摘 要： | 本实验夹闭雄性ＳＤ大鼠冠状动脉左前降支（ＬＡＤ）造成急性心肌缺血,观察ＬＡＤ闭塞后１ｈ内缺血性心律失常的发生。在ＬＡＤ夹闭前２ｈ,静脉注射本室设计的人内皮素－１ｍＲＮＡ反义寡核苷酸（ＡＳ－ＯＤＮ）以阻断ＥＴ－１ｍＲＮＡ表达,观察ＡＳ－ＯＤＮ对血浆ＥＴ－１浓度和急性缺血性心律失常的影响。
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| 关 键 词： | 内皮素 反义寡核苷酸 心肌缺血 心律失常 |
Preventive effect of endothelin-1 mRNA antisense oligonucleotide on acute myocardial ischemic arrhythmia in rats |
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| Lin L,Yuan WJ,Chu XL,Xu H,Li L,Ren AJ.Preventive effect of endothelin-1 mRNA antisense oligonucleotide on acute myocardial ischemic arrhythmia in rats[J].Acta Physiologica Sinica,1999,51(5):533-540. |
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| Authors: | Lin L Yuan W J Chu X L Xu H Li L Ren A J |
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| Institution: | Department of Physiology, Second Military Medical University, Shanghai 200433. |
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| Abstract: | Acute myocedal ischenda was induced by occlusion of the anterior descending ofleft coromp artery (LAD) in rats; the restiltant arrhythmia in 1 h after LAD occlusionwas evaluated. In order to prevent expression of endothelin-1 mRNA, human endothelin-1 mRNA antisense oligonucleohde (ET-1 AS-ODN) was intravenously injected 2 h beforeLAD occlusion. The effect of ANODN on plasrna ET-1 concentheion and the acuteischendc arrhythmia were observed. The results showed tha plasrna ET-1 was significanilydecreased in rats prebeated with ANODN, and both the incidence and severity of theacute ischehac arrhythmia were decreased dose-dependenily as compared with nonnalsaline conbol and sense oligonucleohde conbol, indicating tha ET-1 AS-ODN cotildprevent acute myocedal ischendc apa and tha endogenous endotheIin-1 may playan important role in the devefopment of acute ischendc arrhythmia in rats. |
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| Keywords: | endothelin antisense oligonucleotide myocardial ischemia arrhythmia |
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