The separation of amino acids on ion-exchange resins by elution with water and aqueous ethanol |
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| Authors: | THOMPSON J F MORRIS C J |
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| Affiliation: | 1. State Key Laboratory of Complex Nonferrous Metal Resources Clean Utilization, Kunming University of Science and Technology, Kunming 650093, China;2. National Engineering Laboratory of Vacuum Metallurgy, Kunming University of Science and Technology, Kunming 650093, China;1. Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University, Aligarh 202002, U.P., India;2. Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India;3. Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, U.P., India. |
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| Abstract: | The elution pattern of amino acids from columns of ion-exchange resins in various forms has been studied. A sulfonic acid resin in the sodium form, a carboxylic acid resin in the acid form, and quaternary ammonium resin in the chloride form give the most useful separations. Water and 25% alcohol were the solvents employed. Acidic, basic, and neutral non-alpha amino acids were most highly purified. Partial resolution of neutral α-amino acids, β-alanine, and γ-aminobutyric acid and the basic amino acids were obtained on Dowex 1 in the chloride form.These procedures are characterized by relatively high capacity under mild conditions and have been useful in isolation of three nitrogen compounds from plants. |
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