| Abstract: | A complete series of even-carbon chain polyenoic fatty acids having 20-36 carbons occur in dipolyunsaturated molecular species of phosphatidylcholine from bovine retina. Using oxidative ozonolysis, it is shown that very long chain tetraenes belong to the n-6 series, hexaenes to the n-3 series, and major pentaenes to the n-3 series of fatty acids (very long chain n-6 pentaenes also occur). Molecular ions are obtained by electron impact mass spectrometry of methyl ester derivatives which conclusively identify the major components of this novel group of fatty acids. Mass spectral patterns are similar for the major very long chain tetraenes, for the pentaenes, and for the hexaenes, but different for each group of unsaturation. Very long chain (C24 to C36) polyenes account for about half the weight (40 mol %) of the acyl chains of major dodecaenoic, undecaenoic, and decaenoic molecular species of bovine retina phosphatidylcholine, the other half being made up by docosahexaenoate (22:6 n-3). |
