Rapid purification of human placental aldose reductase |
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Authors: | P F Kador D Carper J H Kinoshita |
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Institution: | Department of Medicine, New York University School of Medicine, 550 First Avenue, New York, New York 10016 USA |
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Abstract: | Sixty percent methanol is widely used for the extraction of nucleotides from lymphocytes for quantitation by high-performance liquid chromatography. In the course of such studies, we noted that these extracts analyzed on an anion-exchange column showed a major “unknown” uv-absorbing peak which eluted after the nucleosides and before the nucleotides. The material cochromatographed with and had the spectral properties of ascorbic acid. This compound was identified as ascorbic acid by chemical and enzymatic assays. The ascorbate content of human lymphocytes determined by high-performance liquid chromatography, cells (mean ± SEM), agreed closely with the levels obtained by standard less sensitive methodology. Evidence is presented that this technique can be used to determine the ascorbate content of lymphocytes where only scanty material or very low levels are found. |
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Keywords: | To whom all communications should be addressed |
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