PRIMARY STRUCTURE OF ALLATOSTATIN 4, A NEVROPEPTIDE INHIBITOR OF JUVENILE HORMONE SYNTHESIS |
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Authors: | Xuechen Guan M. A. Khan |
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Affiliation: | Institute of Zoology, Academia Sinica;, Beijing 100080, China Department of Zoology, University of Toronto;, Toronto, Ontario, M5S 1A1, Canada |
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Abstract: | Abstract Isolation and identification of allatostatin 4 (AST 4) from crude brain extracts of female Diploptera punctata are described. Synthetic analogues of allatostatin 4 truncated from the N-terminal were evaluated to gain some knowledge of structure-activity correlation. AST 4 reversibly inhibits the biosynthesis of JH in vitro . AST 4 has the following sequence: Asp-Arg-Leu-Tyr-Ser-Phe-Gly-Leu-NH2. This neurohormone (AST 4) has no sequence similarity with any known neuropeptide from other organisms. Synthetic AST 4 as well as truncation fragments, including two with the five and six amino acids terminal residues deleted showed in vitro activity distinguishable from that of the native allatostatin. |
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Keywords: | Diploptera punctata corpora allata allatostatin neuropeptides juvenile hormone |
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