Ethanol production from sweet sorghum juice in batch and fed-batch fermentations by Saccharomyces cerevisiae |
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Authors: | Lakkana Laopaiboon Pornthap Thanonkeo Prasit Jaisil Pattana Laopaiboon |
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Affiliation: | (1) Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand;(2) Fermentation Research Center for Value Added Agricultural Products, Khon Kaen University, Khon Kaen, 40002, Thailand;(3) Department of Agronomy, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand |
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Abstract: | Sweet sorghum juice supplemented with 0.5% ammonium sulphate was used as a substrate for ethanol production by Saccharomyces cerevisiae TISTR 5048. In batch fermentation, kinetic parameters for ethanol production depended on initial cell and sugar concentrations. The optimum initial cell and sugar concentrations in the batch fermentation were 1 × 108 cells ml−1 and 24 °Bx respectively. At these conditions, ethanol concentration produced (P), yield (Y ps) and productivity (Q p ) were 100 g l−1, 0.42 g g−1 and 1.67 g l−1 h−1 respectively. In fed-batch fermentation, the optimum substrate feeding strategy for ethanol production at the initial sugar concentration of 24 °Bx was one-time substrate feeding, where P, Y ps and Q p were 120 g l−1, 0.48 g g−1 and 1.11 g l−1 h−1 respectively. These findings suggest that fed-batch fermentation improves the efficiency of ethanol production in terms of ethanol concentration and product yield. |
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Keywords: | Batch fermentation Fed-batch fermentation Ethanol production Saccharomyces cerevisiae Sweet sorghum |
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