番杏Actin基因片段的克隆及生物信息学分析 |
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作者姓名: | 叶玉妍 梁海峰 杨礼香 |
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作者单位: | 广州大学生命科学学院 |
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基金项目: | 国家重点研发计划项目(2016YFC1403002);中国科学院A类战略性先导科技专项(XDA13020500);“十二五”农村领域国家科技计划项目(2015BAL04B04) |
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摘 要: | 本实验为研究番杏(Tetragonia tetragonioides)功能基因表达模式提供内参基因,根据登录在NCBI上植物的Actin基因的保守区域设计简并性引物,通过RT-PCR克隆获得番杏的Actin基因片段,将该片段连接于载体pGEM-T后进行测序,并将该基因序列通过生物信息学软件进行分析。结果显示,克隆所得基因片段大小为598 bp,编码198个氨基酸;该序列与登录在NCBI上的其他植物的Actin基因的核苷酸序列的同源性最大可达86%以上,编码蛋白的氨基酸序列同源性在88%以上。结果表明,本研究克隆所得的基因序列为番杏Actin基因片段。该基因命名为TtActin1,在Gen Bank的登录号为MH33308。
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关 键 词: | 番杏 肌动蛋白 序列分析 |
收稿时间: | 2018/7/18 0:00:00 |
修稿时间: | 2018/8/28 0:00:00 |
Cloning and bioinformatics analysis of Actin gene fragment from Tetragonia tetragonioides |
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Authors: | YE Yuyan LIANG Haifeng YANG Lixiang |
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Institution: | College of Life Sciences, Guangzhou University, Guangzhou 510006, Guangdong, China |
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Abstract: | In order to provide reference gene for studying the expression pattern of functional genes in Tetragonia tetragonioides, the cDNA fragment of Actin from Tetragonia tetragonioides was amplified by RT-PCR, by adopting degenerated primers designed according to the conserved region of the Actin genes which were submitted to NCBI database. The cDNA fragment was inserted into the pGEM-T vector and sequenced. The cDNA sequence was analyzed by online bioinformatics software. Result showed that this Actin cDNA includes 598 base pairs, encoding a partial actin with 198 amino acids. Sequence alignments showed that it shares over 86% nucleotide sequence identity and 88%amino acid sequence identity with other plant actins. This study indicates that a new Actin cDNA fragment is isolated from Tetragonia tetragonioides. This cDNA is named TtActin1 and submitted to GenBank(accession number: MH33308). |
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Keywords: | Tetragonia tetragonioides actin sequence analysis |
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