乙烯对绞股蓝皂苷生物合成关键酶基因表达和皂苷含量的影响

以药食两用植物绞股蓝为材料,研究了植物激素乙烯对绞股蓝皂苷生物合成关键酶基因表达及皂苷含量的影响.该文采用荧光定量PCR技术,检测了乙烯利处理后绞股蓝不同器官中皂苷生物合成关键酶基因GpFPS、GpSS和GpSE的表达水平;采用分光光度法及HPLC技术,测定了乙烯利处理对绞股蓝总皂苷和皂苷单体Rb1、Rb3和Rd含量的...

Effects of ethylene on gypenoside biosynthesis-related key enzyme gene expression and gypenoside content in Gynostemma pentaphyllum

The effects of plant hormone ethylene on gypenoside biosynthesis-related key enzyme gene expression and gypenoside content in medicine-food plant Gynostemma pentaphyllum were analyzed in this study. Fluorescence quantitative PCR was employed to test the expression levels of GpFPS, GpSS and GpSE, key enzyme genes in saponin biosynthesis pathway in different organs of G. pentaphyllum treated with ethephon, while spectrophotometry and HPLC were used to assay the contents of total gypenoside and saponin monomer Rb₁, Rb₃ and Rd in G. pentaphyllum. The results were as follows:(1)The expression levels of GpFPS, GpSS and GpSE genes were up-regulated by addition of ethephon in different extents, and the expression patterns of these three genes were dissimilar in different organs, but similar in the same organ.(2)The contents of total gypenoside in all organs increased when compared with the control, and those in root, mature leaf and young leaf arrived at significant level 3 d after ethephon treatment; However, the three saponin monomers increased or decreased in organs were inconsistency, with Rb₃ had the highest content. The results provide reference for the research of using plant hormone to regulate the secondary metabolism in G. pentaphyllum.