NaCl胁迫对小黄花菜生长及相关生理指标的影响

为探讨小黄花菜的耐盐机理,选育良好的耐盐植物以缓解土壤盐渍化问题,该文选取小黄花菜(Hemerocallis minor)为试材,采用砂培法,研究不同浓度NaCl(50、100、150、200、250 mmol·L-1)胁迫对小黄花菜的生长性状、细胞质膜透性和有机渗透调节物质含量等的影响.结果表明:(1)小黄花菜在10...

Effects of NaCl stress on growth and related physiological indexes in Hemerocallis minor

We explored the salt tolerance mechanism of Hemerocallis minor to be able to select good salt-tolerant plants and alleviate the problem of soil salinization. Using a sand culture method, we analyzed how different concentrations of NaCl stress(0, 50, 100, 150, 200, 250 mmol·L^-1)influence the growth characteristics, cytoplasmic membrane permeability, and organic osmotic adjustment substance content. The results were as followers:(1)The damage to H. minor began at an exposure of 100-150 mmol·L^-1 of NaCl, but did not affect survival. Growth was significantly inhibited at concentrations above 200 mmol·L^-1. Side effects at this concentration included: root system underdeveloping, damaged leaves, and insufficient dry matter accumulation, which seriously affected plant survival status.(2)Under 50-150 mmol·L^-1 of NaCl stress, the cell membrane permeability and MDA content increased slightly. NaCl stress at this concentration range causes limite amounts membrane lipid damage. NaCl stress above 200 mmol·L^-1 makes it impossible to maintain ion balance and the selective permeability of the membrane is lost.(3)we found that as the concentration of NaCl increases, the content of proline in the leaves increased significantly. Under 50-100 mmol·L^-1 of NaCl stress, the soluble sugar content increased at the initial stage of stress, and reached its maximum on 15 d. Soluble sugar content began to decrease at a later period and the soluble protein content in the leaves changed more slowly. This indicated that the main osmotic adjustment substance in H. minor was not soluble protein. Studies have found that H.minor can alleviate salt damage to plants by improving cell membrane permeability and promoting the synthesis of organic osmotic adjustment substances. This allows for H. minor to grow normally in saline-alkali soil under 50-100 mmol·L^-1.