Influence of CW microwave radiation on in vitro release of enzymes from retinol-treated hepatic lysosomes |
| |
Authors: | M. J. Galvin D. P. Parks G. MacNichols D. I. McRee |
| |
Affiliation: | (1) Laboratory of Environmental Biophysics, National Institute of Environmental Health Sciences, P.O. Box 12233, 27709 Research Triangle Park, N.C. |
| |
Abstract: | Hepatic lysosomes were exposed in vitro to microwave radiation (2450 MHz) either prior to or simultaneously with treatment with retinol (vitamin A), and the release of the lysosomal enzymes, β-glucuronidase, acid phosphatase, and cathepsin D, determined. A 60-min microwave exposure (10 or 100 mW/g) of retinol-treated lysosomes had no effect on the amount of release of β-glucuroni-dase, cathepsin D, or acid phosphatase. In addition, 10 and 100 mW/g irradiation of lysosome fractions for 40 min prior to a 20-min retinol and microwave treatment, had no influence on the release of these enzymes. Finally, the effect of microwave radiation on the loss of latency of acid phosphatase and β-glucuronidase from retinol-treated lysosomes was determined. Microwave radiation had no influence on the rate of appearance of these enzymes in the suspending medium. The results indicate that microwave radiation had no effect on the retinol-induced lysosomal enzyme release. |
| |
Keywords: | Microwave, effects on lysosomes radiation, microwave, effects on lysosomes enzymes, microwave effects on release retinol-treated hepatic lysosomes hepatic lysosomes, retinol-treated lysosomes, hepatic, retinol-treated |
本文献已被 SpringerLink 等数据库收录! |
|