Thermal conditioning of fifth-instar Cydia pomonella (Lepidoptera: Tortricidae) affects HSP70 accumulation and insect mortality |
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| Authors: | X YIN S WANG J TANG J D HANSEN S LURIE |
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| Institution: | Department of Biological Systems Engineering, Washington State University, Pullman, Washington, U.S.A.;, College of Plant Protection, Henan Agricultural University, Zhengzhou, Henan, P. R. China;USDA-ARS Yakima Agricultural Research Laboratory, Wapato, Washington, U.S.A.;and Department of Postharvest Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel |
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| Abstract: | Abstract. Levels of HSP70 protein of fifth-instar codling moth Cydia pomonella (L.) (Lepidoptera: Tortricidae)] are determined after conditioning at 35 °C for different times and also after recovery at 22 °C. Protein samples from larvae conditioned for different times are separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis electrophoresis. Sub-lethal thermal conditioning at 35 °C for 40 min, 2, 6 and 18 h induces new protein bands in the extracts from treated codling moth larvae. Immunodetection with an antibody to a heat-inducible HSP70 indicates a stronger reaction after 35 °C for 2, 6 and 18 h than after 35 °C for 40 min or control and, during the recovery period at 22 °C, the level of heat shock protein decreases. Conditioning of fifth-instar codling moths at 35 °C also induces thermotolerance in the insects and necessitates longer times at a lethal temperature to ensure mortality. Thermotolerance is correlated with the accumulation of heat inducible HSP70 protein. |
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| Keywords: | Cydia pomonella fruit pest species heat shock protein pest management preconditioning thermal resistance |
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