8-Nitroxanthine,a product of myeloperoxidase,peroxynitrite, and activated human neutrophils,enhances generation of superoxide by xanthine oxidase |
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Authors: | Yeh George C Henderson Jeffrey P Byun Jaeman André d'Avignon D Heinecke Jay W |
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Institution: | Department of Biological Sciences, University of Pittsburgh, PA 15260, USA. rbentley@pitt.edu |
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Abstract: | In biology, chiral recognition usually implies the ability of a protein, such as an enzyme or a drug receptor, to distinguish between the two enantiomeric forms of a chiral substrate or drug. Both diastereoisomerism and specific contacts between enzyme/receptor and substrate/drug are necessary. The minimum requirement is for four contact points including four nonplanar atoms (or groups of atoms) in both probe and target. The molecular models described by Easson and Stedman and by Ogston require three binding sites in a plane. A modified model with three binding sites in three dimensions is described. Under certain circumstances this model allows binding of both enantiomeric forms of a substrate or a drug. Enantiomer superposition of two enantiomers at an active site occurs in some specific cases (e.g., phenylalanine ammonia-lyase, isocitrate dehydrogenase) and is likely in others. The nature of enantiomer binding to racemase enzymes is discussed. |
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Keywords: | Chiral recognition Role of diastereoisomerism Easson-Stedman model Ogston model Three-dimensional model Enantiomer superposition Nonenantioselective enzymes Racemases |
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