Apoptosis Induction in Human Lymphoma and Leukemia Cell Lines by Transfection via Dendrosomes Carrying Wild-Type p53 cDNA |
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| Authors: | Mohammad Massumi Abed Ali Ziaee Mohammad Nabi Sarbolouki |
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| Institution: | (1) Institute of Biochemistry and Biophysics, Tehran University, P.O. Box 13145-1384, Tehran, Iran;(2) Department of Stem Cells, Royan Institute, P.O. Box 19395–4644, Tehran, Iran |
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| Abstract: | The efficiency of dendrosomes (novel dendritic spheroidal nanoparticle gene porters) were assessed in transferring wild-type
p53 cDNA into two human acute lymphoblastic leukemia cell lines (MOLT-4 and CCRF-CEM) derived from T cells and erythroleukemic
cell line K562. Flow cytometric studies showed a 65% and 45% enhancement in apoptosis and necrosis of K562 and CCRF-CEM cells
transfected with complex of dendrosomes and wild-type p53 cDNA in comparison to controls. The cytotoxicity studies on T lymphoma
cells revealed that dendrosomes have a low cytotoxicity in comparison to lipofectin. |
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| Keywords: | apoptosis dendrosomes flow cytometry lipofectin lymphoblastic leukemia cell lines |
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