Methionine and homocysteine modulate the rate of ROS generation of isolated mitochondria in vitro |
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Authors: | Jose?Gomez Ines?Sanchez-Roman Alexia?Gomez Carlota?Sanchez Henar?Suarez Monica?Lopez-Torres Email author" target="_blank">Gustavo?BarjaEmail author |
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Institution: | Department of Animal Physiology II, Faculty of Biological Sciences, Complutense University, Madrid, Spain. |
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Abstract: | Dietary methionine restriction and supplementation in mammals have beneficial (antiaging) and detrimental effects respectively,
which have been related to chronic modifications in the rate of mitochondrial ROS generation. However it is not known if methionine
or its metabolites can have, in addition, direct effects on the rate of mitochondrial ROS production. This is studied here
for the methionine cycle metabolites S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), homocysteine and methionine
itself in isolated rat liver, kidney, heart, and brain mitochondria. The results show that methionine increases ROS production
in liver and kidney mitochondria, homocysteine increases it in kidney and decreases it in the other three organs, and SAM
and SAH have no effects. The variations in ROS production are localized at complexes I or III. These changes add to previously
described chronic effects of methionine restriction and supplementation in vivo. |
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