马尾松组培生根关键因子分析

该研究以马尾松( Pinus massoniana)优良种群无性化组培继代芽为材料，探讨了影响马尾松组培单芽生根的几个关键因素如基本培养基、基因型、激素及光温条件，所有数据均采用SPSS 19.0统计分析软件进行方差分析及显著性检验。结果表明：(1)以半量的WPM、DCR、GD、MS和改良MS为基本培养基进行生根处理，生根率差异显著，其中以1/2改良MS的生根效果最佳。(2)基因型不同，马尾松组培生根能力差异显著，在供试无性系中，以GLM-80的组培生根能力较强。(3)生根激素的浓度显著影响了马尾松组培生根，低浓度的生长素处理，生根率高、根系发达，与IAA、IBA相比，低浓度0.1~0.2 mg·L-1 NAA处理下的组培生根效果好。(4)光照强度对马尾松组培生根影响不明显，但对芽苗地上部分形态建成影响显著；在2000 lx光照强度处理下，芽苗生长健壮、移栽成活率高，而温度对马尾松组培生根具有显著的影响；在25℃培养条件下，生根效果稳定。该研究结果为马尾松组培苗工厂化生产提供了科技支撑，为实现马尾松无性系育林、加快良种选育进程奠定了坚实基础。

Key factors affecting rooting of Pinus massoniana by tissue culture

Influence of certain selected factors including basal media, genotypes, hormone and conditions of light and temperature on rooting of in vitro single buds was explored using tissue-cultured shoots of good variety population in Pinus massoniana. SPSS 19.0 statistic software was used to analyze those data. Results were as follows:(1) Rooting percentage was significantly different under the treatments of half-strength WPM, DCR, GD, MS and modified MS basic media. The best rooting was observed in the treatment of 1/2 modified MS. ( 2) The difference in rooting ability was significant in genotypes of P. massoniana. Rooting capacity of GLM-80 was stronger than other clones tested. (3) Hormone concentra-tion remarkably affected in vitro rooting of P. massoniana. Rooting percentage was high and root system was developedunder the treatment of low-concentrate auxin. The performance of rooting by tissue culture was good with the treatment of 0.1-0.2 mg·L-1 NAA compared to IAA and IBA. (4) Light intensity did not affect in vitro rooting of P. massoniana, while significantly influenced morphogenesis of aerial parts of shoots. Shoots grew well and transplantation survival per-centage was high when treated with the light intensity of 2 000 lx. There was significant of temperature on in vitro rooting of P. massoniana, and rooting effect was stable under the cultural condition of 25℃. This study provides the scientific and technological support for the industrialized production of tissue cultured plantlets, and for the implementation of clonal forestry as well as the acceleration of the process for improved varieties breeding in P. massoniana.