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Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. |
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| Authors: | A G Ostle and J G Holt |
| Abstract: | Poly-beta-hydroxybutyrate granules exhibited a strong orange fluorescence when stained with Nile blue A. Heat-fixed cells were treated with 1% Nile blue A for 10 min and were observed at an excitation wavelength of 460 nm. Glycogen and polyphosphate did not stain. Nile blue A appears to be a more specific stain for poly-beta-hydroxybutyrate than Sudan black B. |
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