Interference of raw milk autochthonous microbiota on the performance of conventional methodologies for Listeria monocytogenes and Salmonella spp. detection |
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Authors: | Nero Luís Augusto de Mattos Marcos Rodrigues Barros Márcia de Aguiar Ferreira Beloti Vanerli Franco Bernadette Dora Gombossy de Melo |
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Affiliation: | aUniversidade Federal de Viçosa, Departamento de Veterinária, Av. P.H. Rolfs s/n, Centro, 36570 000 Viçosa, MG, Brazil;bUniversidade de São Paulo, Faculdade de Ciências Farmacêuticas, Departamento de Alimentos e Nutrição Experimental, Av. Prof. Lineu Prestes, 580, 05508 900 São Paulo, SP, Brazil;cUniversidade Estadual de Londrina, Departamento de Medicina Veterinária Preventiva, Caixa Postal 6001, 86051 990 Londrina, PR, Brazil;dUniversidade de Brasília, Faculdade de Agronomia e Medicina Veterinária, Caixa Postal 4508, 70910 970 Brasília, DF, Brazil |
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Abstract: | Pathogen detection in foods by reliable methodologies is very important to guarantee microbiological safety. However, peculiar characteristics of certain foods, such as autochthonous microbiota, can directly influence pathogen development and detection. With the objective of verifying the performance of the official analytical methodologies for the isolation of Listeria monocytogenes and Salmonella in milk, different concentrations of these pathogens were inoculated in raw milk treatments with different levels of mesophilic aerobes, and then submitted to the traditional isolation procedures for the inoculated pathogens. Listeria monocytogenes was inoculated at the range of 0.2–5.2 log CFU/mL in treatments with 1.8–8.2 log CFU/mL. Salmonella Enteritidis was inoculated at 0.9–3.9 log CFU/mL in treatments with 3.0–8.2 log CFU/mL. The results indicated that recovery was not possible or was more difficult in the treatments with high counts of mesophilic aerobes and low levels of the pathogens, indicating interference of raw milk autochthonous microbiota. This interference was more evident for L. monocytogenes, once the pathogen recovery was not possible in treatments with mesophilic aerobes up to 4.0 log CFU/mL and inoculum under 2.0 log CFU/mL. For S. Enteritidis the interference appeared to be more non-specific. |
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Keywords: | Autochthonous microbiota Milk Pathogen detection Listeria monocytogenes Salmonella spp. |
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