Optimization of medium constituents and fermentation conditions for the production of ethanol from palmyra jaggery using response surface methodology |
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| Authors: | Email author" target="_blank">B?V?V?RatnamEmail author S?Subba Rao Damodara Rao?Mendu M?Narasimha Rao C?Ayyanna |
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| Institution: | (1) Department of Neurology, Johns Hopkins University School of Medicine, Pathology 233/Meyer 222 600 North Wolfe Street, Baltimore, MD 21287, USA;(2) Department of Pediatrucs Division of Infectious Diseases, Johns Hopkins University School of Medicine, 720 Rutland Avenue Ross 1135B, MD 21205 Baltimore, USA;(3) Center for Biotechnology Department of Chemical Engineering, College of Engineering Andhra University, Visakhapatnam, 530 003, India |
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| Abstract: | The quantitative effects of sugar concentration, nitrogen concentration, EDTA, temperature, pH and time of fermentation on ethanol production were optimized using a Box-Wilson central composite design (CCD) experiment. It was found that palmyra jaggery (sugar syrup from the palmyra palm) is a suitable substrate for the production of high concentrations of ethanol using Saccharomyces cerevisiae NCIM 3090 by submerged fermentation. A maximum ethanol concentration of 129.4 g/l was obtained after optimizing media components and conditions of fermentation. The optimum values were a temperature of 26.2 °C, pH of 8.4, time of fermentation of 4.2 days with 398.5 g of substrate/l, 3.1 g of urea/l and 0.51 g of EDTA/l. Thus by using the CCD, it is possible to determine the accurate values of the fermentation parameters where maximum production of ethanol occurs. |
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| Keywords: | Central composite design (CCD) ethanol palmyra jaggery response surface methodology (RSM) Saccharomyces cerevisiae |
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