Enhancing effect of magnesium ion on cell-free synthesis of read-through protein of bacteriophage Qbeta. |
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Authors: | A Hirashima H Harigai I Watanabe |
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Affiliation: | Department of Molecular Biology, Keio University, School of Medicine, Shinjuku-ku, Tokyo 160, Japan |
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Abstract: | This report describes the enhancing effect of magnesium ion on the synthesis of read-through protein of bacteriophage Qβ in a cell-free protein synthesizing system from . At 6 mM of magnesium acetate, the major product was coat protein. At 12 mM of magnesium, it was replaced by read-through protein. This enhanced synthesis was substituted by the addition of 0.25 mM of spermine or 1 mM of spermidine to 6 mM of magnesium. These results suggest that magnesium or combination of magnesium and polyamines causes leaky termination at the end of the coat protein cistron of Qβ-RNA. |
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