| Abstract: | The paper presents ultrastructural aspects related to zygotes and embryo cytodifferentiation of Sus scrofa d., used in the biotechnology of embryo transfer, from the first division of segmentation to the stage of preimplantational blastocyst. Together with the intense cellular proliferations, the morphostructural and functional differentiation of cells takes place. First, the blastomeres are similar from an ultrastructural point of view and do not present intercellular junctions to the advanced stage of morula. Gradually, the polarity of the cellular surface is more evident (microvilli occupy only certain domains of the cellular surface and junctions of intercellular solidarity of desmosomal type occur). Ultrastructural modifications of nuclei, mitochondria and cytoskeleton are noticed. Embryoblastic cells as well as those to form the trophoblast are morphostructurally and positionally differentiated. In the stage of preimplantational blastocyst, the nuclei of some embryoblastic cells have a characteristic form of a callotte or biconcave lens with passages of nuclear material to the cytoplasm. |
