棉蚜获毒后禁食对其保持并传播黄瓜花叶病毒的影响

采用棉蚜Aphis gossypii 甜瓜Cumumis melo 黄瓜花叶病毒（cucumber mosaic virus, CMV）体系，研究棉蚜获毒后在空气中禁食对其保持并传播黄瓜花叶病毒的影响。结果表明获毒后的禁食时间与棉蚜传毒效率呈负相关。运用EPG (electrical penetration graph)及其即时显示、即时中断技术研究分析棉蚜禁食后的早期传毒行为细节。结果显示：禁食处理没有显著影响电势落差（potential drop，pd）数目及穿刺过程中出现的第一个pd波形前穿刺时间这两个重要指标，但禁食处理能引起pd波的两个亚波形pdⅡ-1和pdⅡ-2持续时间的显著减短。进一步分析未禁食棉蚜传毒作用与pd亚波形的关系，显示传毒可能与pdⅡ-2的持续时间相关（P＝0.06）。因此，pdⅡ-2的持续时间可能是与棉蚜传毒相关的一个行为指标。该研究还建立了新的高效而稳定的获毒方法---5pd获毒法，与传统的5min获毒法相比，获毒效率显著提高。

Effects of post-acquisition fast on cucumber mosaic virus transmission by the cotton aphid, Aphis gossypii

Viruliferous cotton aphids, Aphis gossypii, were treated with post-acquisition fast in the air to study cucumber mosaic virus (CMV) retention in the aphid and consequent transmission on the melon, Cumumis melo. Transmission efficiency was negatively correlated with fasting duration. The 10-min inoculation processes of fasted and non-fasted aphids were studied with electrical penetration graph (EPG) technique to search the behavior events responsible for the reduction of transmission efficiency. The total number of potential drops and the probing time before the 1st pd were usually considered as two important behavior variables correlated with transmission efficiency; however, no significant difference was found between fasted and non-fasted aphids. Fasting treatment significantly decreased the durations of sub-phase pdⅡ-1and pdⅡ-2. Further analysis suggested that the duration of pdⅡ-2 was probably a behavioral factor correlated with the virus transmission. The related behavioral mechanism was discussed. The 5 pd acquisition method was first used in virus acquisition research and a significantly higher transmission efficiency (78.6% vs 46.40%, P＜0.0001) was obtained compared to the traditional 5 min acquisition method.