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Bombesin binding and biological effects on pancreatic acinar AR42J cells
Authors:C D Logsdon  J C Zhang  J Guthrie  S Vigna  J A Williams
Institution:1. Cell Biology Laboratory and the Department of Medicine, Mount Zion Hospital and Medical Center and the Department of Physiology University of California San Francisco, San Francisco CA 94120, USA;1. The Geological Survey of Denmark and Greenland, Øster Voldgade 10, 1350 Copenhagen K, Denmark;2. Geology Department, Ministry of Mineral Resources, Government of Greenland, P.O. Box 930, 3900 Nuuk, Greenland;3. Centre for Exploration Targeting – Curtin Node, The Institute for Geoscience Research, John de Laeter Centre, School of Earth and Planetary Sciences, Curtin University, Perth, Western Australia 6102, Australia;4. Department of Geosciences and Natural Resource Management, University of Copenhagen, Øster Voldgade 10, 1350 Copenhagen K, Denmark;5. Department of Earth and Environmental Sciences, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada;6. School of Earth and Environmental Sciences, University of St. Andrews, St. Andrews KT16 9AL, United Kingdom;1. Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, University of Campania-L.Vanvitelli, ex Second University of Naples, Via De Crecchio 7, 80138, Naples, Italy;2. Department of Chemical Sciences, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy;3. Department of Biology, University of Naples Federico II, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126, Naples, Italy;1. Fuel Fabrication, INRP(O), Bhabha Atomic Research Centre, Tarapur, Maharashtra 401502, India;2. Post-Irradiation Examination Division, Indira Gandhi Centre for Atomic Research, Kalpakkam, Tamil Nadu 603102, India;1. Department of Industrial Engineering and Engineering Management, National Tsing Hua University, Hsinchu 30013, Taiwan;2. Department of Project Management, The University of Danang – University of Science and Technology, Danang, Viet Nam;3. Department of Industrial Management, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan
Abstract:The effects of bombesin on amylase release and the receptor binding of 125I-Tyr4]bombesin in the rat pancreatic acinar carcinoma cell line AR42J were examined. Bombesin-like peptides stimulated amylase release from AR42J cells in a dose-dependent manner; a maximal 2-fold stimulation occurred at a bombesin concentration of 300 pM. Binding of 125I-Tyr4]-bombesin to AR42J cells was specific, saturable and temperature dependent. The relative potencies with which various structurally related peptides stimulated amylase release correlated well with their relative abilities to compete for the bombesin receptor.
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