The incorporation of [myo-2-3H] inositol into phosphatidyl inositol of stimulated rat pancreas |
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| Authors: | B A Chapman H Patapanian N R Pattinson J S Wilson R C Pirola J B Somer |
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| Institution: | Department of Medicine, University of New South Wales, Prince Henry Hospital, Little Bay, Australia. |
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| Abstract: | The 'phospholipid effect' involves agonist induced breakdown of phosphatidyl inositol (PI) or its phosphorylated derivates with increased incorporation of 32P or myo-2-3H] inositol during resynthesis. In rat pancreas pancreozymin and bethanecol resulted in the standard dose dependent increased incorporation of 32P into PI which was paralleled by increased amylase secretion. By contrast the incorporation of myo-2-3H] inositol into PI was significantly decreased by pancreozymin whereas bethanecol had no effect. However, pancreozymin caused a 30% decrease in labelled PI irrespective of whether it was prelabelled with 32P or myo-2-3H] inositol. Thus in rat pancreas, pancreozymin resulted in the standard agonist induced breakdown of pre-labelled PI but inhibited the incorporation 2-3H-myo] inositol during the resynthetic phase. |
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