Construction of a novel cell-surface display system for heterologous gene expression in Escherichia coli by using an outer membrane protein of Zymomonas mobilis as anchor motif |
| |
Authors: | Ming-xiong He Hong Feng Yi-zheng Zhang |
| |
Institution: | (1) Sichuan Key Laboratory of Molecular Biology & Biotechnology, Key Laboratory of Resource Biology & Eco-environment of Ministry of Education, College of Life Science, Sichuan University, Chengdu, 610064, China |
| |
Abstract: | A novel bacterial cell-surface display system was developed in Escherichia coli using omp1, a hypothetical outer membrane protein of Zymomonas mobilis. By using this system, we successfully expressed β-amylase gene of sweet potato in E. coli. The display of enzyme on the membrane surface was also confirmed. The recombinant β-amylase showed to significantly increase
hydrolytic activity toward soluble starch. Our results provide a basis for constructing an engineered Z. mobilis strain directly fermenting raw starch to produce ethanol. |
| |
Keywords: | β -Amylase Cell-surface display Outer membrane protein Sweet potato Zymomonas mobilis |
本文献已被 PubMed SpringerLink 等数据库收录! |