The Use of Density Gradient Centrifugation for the Separation of Germinated from Nongerminated Spores |
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| Authors: | G A Prentice F H Wolfe L F L Clegg |
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| Institution: | Department of Food Science, University of Alberta, Edmonton, Canada |
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| Abstract: | S ummary : The density gradient centrifugation of a suspension of spores of B. subtilis 8057 on both sucrose and renografin gradients gave 2 distinct fractions. Germination evidence suggested that the heavier fraction consisted of dormant spores and the less dense fraction, germinated spores. It is concluded that density gradient centrifugation may provide a useful technique for the separation of germinated from nongerminated spores. |
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