| 曲格列酮对THP-1巨噬细胞源性泡沫细胞ABCA1表达及胆固醇流出的影响 |
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| 引用本文： | 甘淋,彭家和,鲁立,申丽丽,张迁,江渝. 曲格列酮对THP-1巨噬细胞源性泡沫细胞ABCA1表达及胆固醇流出的影响[J]. 现代生物医学进展, 2007, 7(1): 82-84 |
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| 作者姓名： | 甘淋 彭家和 鲁立 申丽丽 张迁 江渝 |
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| 作者单位： | 第三军医大学生物化学及分子生物学教研室,重庆,400038 |
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| 摘 要： | 目的:研究曲格列酮对THP-1巨噬细胞源性泡沫细胞ABCA1表达及胆固醇流出的影响。方法:采用液体闪烁计数法测定曲格列酮处理后THP-1巨噬细胞源性泡沫细胞胆固醇流出。用RT-PCR和Western blotting的方法检测曲格列酮处理后THP-1巨噬细胞源性泡沫细胞ABCA1 mRNA水平和蛋白质水平的变化。结果:经曲格列酮处理后,THP-1巨噬细胞源性泡沫细胞的胆固醇流出具有时间依赖性的增加,从0h的1．82%上升到24h的7．61%。在mRNA水平和蛋白质水平ABCA1也均随着曲格列酮作用时间增加而表达上调。结论:曲格列酮能增加THP-1巨噬细胞源性泡沫细胞ABCA1表达和胆固醇流出。
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| 关 键 词： | 胆固醇流出 PPARγ |
| 文章编号： | 24081140 |
| 修稿时间： | 2006-10-10 |
Effects of Troglitazone on ATP Binding Cassette Transporter A1 Expression and Cholesterol Efflux in THP-1 Macrophage- derived Foam Cells-derived Foam Cells |
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| GAN Lin,PENG Jia-he,LU li,SHEN Li-li,ZHANG Qian,JIANG Yu. Effects of Troglitazone on ATP Binding Cassette Transporter A1 Expression and Cholesterol Efflux in THP-1 Macrophage- derived Foam Cells-derived Foam Cells[J]. Progress in Modern Biomedicine, 2007, 7(1): 82-84 |
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| Authors: | GAN Lin PENG Jia-he LU li SHEN Li-li ZHANG Qian JIANG Yu |
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| Affiliation: | Department of Biochemistry and Molecular Biochemistry, The Third Military Medical University, ChongQing, 400038, China |
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| Abstract: | Objective:To study the effect of Troglitazone on ATP binding cassette transporter A1(ABGA1)expression and cholesterol efflux in THP-1 macrophage-derived foam cellss.Methods:After exposure of the cultured THP-1 macrophage- derived foam cells in Troglitazone for different times,cholesterol efflux was determined by liquid scintillator.ABCA1 mRNA and its protein level were determined by RT-PGR and Western blotting respectively.Results:The results showed that Troglitazone markedly increased ABCA1-mediated cholesterol efflux from THP-1 macrophage-derived foam cells,from Oh 1.82% to 24h 7.61%.This was accompanied by an increase membrane content of ABCA1,either in the level of protein or of mRNA. Conclusion:Troglitazone increases cholesterol efflux and the level of ABCA1 protein and mRNA in THP-1 macrophage-derived foam cells |
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| Keywords: | ABCA1 AS |
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